The presence of specific sensibilization of lymphocytes of the patients to liver tissue was examined by blast-transformation test. For this purpose they compared immunomorphologic response to lymphocytes in culture from peripheral blood to liver and renal antigen, using parallel procedure with FHA and cultures, growing spontaneously. Ten clinically healthy and 12 persons with renal polycystosis were examined as controls.
The results showed an increased immune response to lymphocytes of the patient with liver cirrhosis to liver antigen as well as to culture, developing spontaneously, which suggested that autoimmune reaction with specific tendency to antigens of liver developed in these persons.
cristatus of 16 antibiotics (keflin, penicillin, benzazillin, ampicillin, nystatin, kanamycin, streptomycin, novobiocin, brunomycin, erythran, oleandomycin, tetracyclin, chlornytromycin, gentamycin, rimactan, and capreomycin) administered in 3 concentrations was studied.
It was established that except kanamycin, keflin and rimactan all the rest damage the embryonic development showing certain phase specifity. The authors carried out experiments on mice DBA/2 and BDF1 with leucosis L1210 and lung carcinoma of Lewis and found that psycotropic preparations tempidone and caffeine did not manifest antitumorous effect and toxicity after the used doses and scheme of treatment.
Tempidone enhanced antitumorous action of small and mean doses of cyclophosphamide in mice with leucosis L1210. In the triple combination (tempidone, caffeine and cycl phosamide) the caffeine enhanced this action of tempidone without raising toxicity in mice with leucosis L1210, but not in mice with lung carcinoma of Lewis.
In order to elucidate the positive feedback mechanism of estrogen on gonadotropin release in women, the responses of plasma LH and FSH to the constant infusion of estradiol-17 beta for a prolonged period were studied. The infusion was initiated on various days of the follicular phase and maintained for 36-66 hr at a constant rate of 500 or 1,000 microgram/24 hr.
When the stimulus of estradiol was sustained for more than 30 hr in the women of the middle or late follicular phase, a positive feedback effect to elicit gonadotropin surges was observed during the maintenance of the infusion. In contrast, the stimulus of estrogen was ineffective in the early follicular phase, even if sustained for a longer period up to 66 hr.
The magnitude of the responses, however, was much smaller, as compared to spontaneous preovulatory gonadotropin surges. In all cases, the effect of estradiol was greater for LH than for FSH. It is suggested that: 1) Preovulatory gonadotropin surges are triggered by estrogen increments rather than the withdrawal of the negative feedback effect of estrogen.
2) Low levels of estrogen for a certain period of the early follicular phase may play an important role in priming the control system which responds to the positive feedback effect of estrogen. Nalpha-Tyrosyl-somatostatin was synthesized and proved to be homogeneous.
Radioiodination of this tyrosine-containing somatostatin analogue by either the lactoperoxidase method or the chloramine T method led to the formation of crude iodinated compound, which was purified by ion exchange chromatography on CM-Sephadex C-25 using a linear ammonium acetate buffer gradient. This purification process was found to be satisfactorily reproducible and suitable for the preparation of 125I-Nalpha-tyrosyl-somatostatin.
Using the purified 125I-somatostatin analogue, radioimmunoassay for somatostatin was performed and the assay system was proved to be sensitive and specific for somatostatin. Immunoassays of hot-water extracts of porcine and tupaia brain, pancreas, stomach and various regions of the intestine in the system revealed that those tissues contained immunoreactive somatostatin at various concentrations.